Table 1.

Nucleotide sequences of the primer-pairs used for conventional RT-PCR

Primer Name	Gene	Isoform	Primer/Probe	Sequence	Reference
Gen TPM1-1α&κ(+) Gen TPM1-1α&κ(−) TPM1-1α. Pro.(+) TPM1-1κ Primer (+) TPM1-1κ Pro (+)	TPM1-1 TPM1-1 TPM1-1 TPM1-1 TPM1-1	TPM1-1α & κ TPM1-1α & κ TPM1-1α TPM1-1κ TPM1-1κ	Primer Primer Probe Prime Probe	5′-CATGGATGCCATTAAGAAGAA-3′ 5′-AAGTCATGTCATTGAGAGCG-3′ 5′-CTTGAAGATGATCTGCTCGCAC-3′ 5′-TAGAAGACGAACTAATTCAG-3′ 5′-GGAAAAGAGGTTGCGCGTG-3′
TPM1-2α (+) TPM1-2α (−) TPM1-2ν(−)	TPM1-2 TPM1-2 TPM1-2	TPM1-2α TPM1-2α TPM1-2ν	Primer Primer Primer	5′-ACTCACACAGCCATGGAT-3′ 5′-GCCCGGAGCCGAGATGAGATGT-3′ 5′-TGGTCTTCTCAAGCTTGGCCTCCT-3′	M24635.1 M24635.1 This study
TPM2α(+) TPM2α(−)	TPM2 TPM2	TPM2α TPM2α	Primer Primer	5′-ATGGAGGCCATCAAGAAGAA-3′ 5′-AGACAGAGGAGACCGAAAGAGT-3′
TPM3α (+) TPM3α(−)	TPM3 TPM3	TPM3α TPM3α	Primer Primer	5′-GTAACACATTAAAATGGCCGGAT-3′ 5′-TTAGAAACTATTGAGCTCGCTCA-3′
TPM4-2(+) TPM4-2(−) TPM4-3(+)	TPM4-2 TPM4-2 TPM4-2	TPM4-2α TPM4-2α	Primer Primer for Nested PCR	5′-TCCCTCACAGCAGCACAA-3′ 5′-CACAGATTCTGATTCAGTTATA-3′ 5′-ATGGAGGCCATCAAGAAGAA

Name	Gene	Isoform	Primer/Probe	Sequence	Reference
TPM4-1α (+) TPM4-1α (−) TPM4-1.Total(+) TPM4-1.Total(−) TPM4-1.Probe	TPM4-1 TPM4-1 TPM4-1 TPM4-1 TPM4-1	TPM4-1α TPM4-1α Total transcripts Total transcripts	Primer +ve Primer −ve Primer +ve Primer −ve Probe	5′-CCATGGAGGCCATCAAGAA-3′ 5-CAGGGATGTCGTGTCATTGAGAGCATGA-3′ 5′-GCCCTGAACCGCAGGATT-3′ 5′-ATTTTTCCAGTTTGGCCACTGT-3′ 5′-AGAAGACAAGTATGAAGAAG-3′	NM001024467 NM_001024467 NM_001024467 NM_001024467 NM_001024467