Table 2.

Nucleotide sequences of the primer-pairs used for qRT-PCR

Name	Gene	Isoform	Primer/cDNA	Sequence	Reference
Zb.Fish.TPM4-1α (+) Zb.Fish.TPM4-1α (−) Zb.Fish.TPM4-1Ex9A(−)	TPM4-1 TPM4-1 TPM4-1	TPM4-1α TPM4-1α TPM4-1	Primer Primer cDNA	5′-AGAAGACAAGTATGAAGAAG-3′ 5′-GTAGAGCGAGACAGATGGAT-3′ 5′CAGGGATGTCGTGTCATTGAGAGCATGA-3	BC085518 BC085518 BC085518
Zb.Fish. TPM4-2α (+) ZB.Fish. TPM4-2α(−) ZB.Fish.TPM4-2 Ex9A(−)	TPM4-2 TPM4-2 TPM4-2	TPM4-2α TPM4-2α TPM4−2	Primer Primer cDNA	5′-AAGAGGACAAATATGAGGAT-3′ 5′-ATACCATCTATAGGCACTCAT-3′ 5′-CACAGTTCTGATTCAGTTATA-3′
Zb.Fish.TPM1-1α(+) Zb.Fish.TPM1-1α(−) Zb.Fish. TPM1-1κ(+) Zb.Fish. TPM1-1κ(−) Zb.Fish.TPM1-1Ex9A(−)	TPM1-1 TPM1-1 TPM1-1 TPM1-1 TPM1-1	TPM-1α TPM-1α TPM-1κ TPM-1κ TPM-1α&κ	Primer Primer Primer Primer cDNA	5′-AGAAAGCTGCGGAGGACAAAT-3′ 5′-TCCTCCACCAGCTGAATCCTG-3′ 5′-CAGTTAGAAGACGAACTAA-3′ 5′-ACATCACCTTCAGCCTTG-3′ 5′-AAGTCATGTCATTGAGAGCG-3′	NM_001024467.1 NM_001024467.1 This study This study NM_001024467.1
Zb.Fish.TPM1-2α(+) Zb.Fish.TPM1-2α(−) Zb.Fish.TPM1-2ν (+) Zb.Fish.TPM1-2ν (−)	TPM1-2 TPM1-2 TPM1-2 TPM1-2	TPM1-2α TPM1-2α TPM1-2NeW TPM1-2New	Primer Primer Primer Primer	5′-TGACTGACAAGCTGAAGGAGG-3′ 5′-GCCCGGAGCCGAGATGAGATGT-3′ 5′-CCCTGAACAGACGCATCCAGC-3′ 5′-TGGTCTTCTCAAGCTTGGCCTCCT-3′	M24635.1 M24635.1 This study M24635.1 M24635.1
Zb.Fish. 18S.rRNA (+) Zb.Fish. 18S.rRNA (−)	18s rRNA 18s rRNA	- -	Primer Primer	5′-GTGGAGCGATTTGTCTGGTT-3′ 5′-TTATGACCTGCGCTTACTGG-3′	FJ915075.1 FJ915075.1