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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Chem Neuroanat. 2016 Sep 15;83-84:99–106. doi: 10.1016/j.jchemneu.2016.09.006

Fig. 6. Inhibitory effects of decynium 22 and GBR12935 on hPMAT-, rPmat- and mPmat-mediated uptake of MPP+.

Fig. 6

The hPMAT- (closed circles) and rPmat- (closed triangles) mediated uptake of 1 µM MPP+ as only [H3]-labeled ligands was measured in the absence or presence of various concentrations of (A) decynium 22 or (C) GBR12935 for 3 min at 37 °C and pH 7.4. The mPmat- (closed squares) mediated uptake of MPP+ (1 µM) was measured in the absence or presence of various concentrations of (B) decynium 22 or (D) GBR12935 for 2 min at 37 °C and pH 7.4. The hPMAT-, rPmat- and mPmat-mediated uptake was determined by subtracting the uptake by MDCK/mock cells from that by MDCK/hPMAT, MDCK/rPmat and Flp293/mPmat cells, respectively. The inhibitory effects of decynium 22 or GBR12935 on hPMAT-, rPmat- and mPmat-mediated uptake of MPP+ are presented as percent of control. Data are shown as the mean ± SEM (n = 3).