Fig. 2.

Effects of ethanol exposure on ALDH2 mRNA and protein expressions in rat primary cardiomyocytes. The cells were exposed to different concentrations of ethanol (0.1, 0.3, and 0.5%) for 4 days and subjected to detection. a ALDH2 mRNA expression was analyzed by RT-PCR. GAPDH was used as an internal control. b ALDH2 protein expression was analyzed by western blot. GAPDH was employed as a loading control. Relative ALDH2 protein expression to control in each group was shown in c. Data were presented as mean ± S.D. **p < 0.01 compared to control (no ethanol treatment)