Fig. 3.

Ethanol (0.5%) inhibits ALDH2 expressions through miR-378a-5p in rat primary cardiomyocytes. a 18 μ-RNAs predicted to target ALDH2 were found to be upregulated or downregulated by ethanol treatment by microarray analysis. b Level of miR-378a-5p was analyzed by mature miRNA assay in rat primary cardiomyocytes exposed to ethanol. c ALDH2 mRNA level in the cells transfected with either negative control miR (miR-NC) or miR-378a-5p. mRNA levels were analyzed by RT-PCR. GAPDH was used as an internal control. d ALDH2 protein expression level was examined by western blot analysis. GAPDH was employed as a loading control. Data were presented as mean ± S.D. **p < 0.01 compared to control (no ethanol treatment)