FIG. 4.
Flavonoids reduce FM 1-43FX uptake by hair cells. a Mean relative fluorescence (normalized to the appropriate vehicle-only control and quantified in arbitrary units) of all images taken for each flavonoid at 50 μM concentration (24-h exposure) demonstrates a significant reduction in fluorescent intensity (one-way ANOVA, F4, 86 = 4.331, p = 0.003). **p < 0.01. N = 20–24, bars are +S.E.M. b Representative images showing FM 1-43FX fluorescence in control (EtOH) and treated neuromasts. Top images show FM 1-43FX labeling, while bottom panels show DIC images for the same neuromast. The scale bar in the top left image is 10 μm and applies to all images. Brightness was equally increased by 60 % across all images, and the images were converted to grayscale to increase visibility for print; no changes were made to the images used for fluorescence quantification.