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VGCC and NSCC contribute to PE-mediated contractions at 16 and 40 mN. PE concentration-response curves for isometric contractions at preloads of 40 mN (black) or 16 mN (white) in the absence (A,C) and presence (B,D) of 300 μM L-NAME were due to Ca2+ influx via VGCC and NSCC. Isometric contractions in the presence of 1 μM levcromakalim reveal contractions due to Ca2+ influx via NSCC only (C,D). *p < 0.05, **p < 0.01, ***p < 0.001, 40 vs. 16 mN preload (n = 4).
