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. 2017 Mar 16;7:77. doi: 10.3389/fcimb.2017.00077

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Copyright © 2017 Ngba Essebe, Visvikis, Fines-Guyon, Vergne, Cattoir, Lecoustumier, Lemichez, Sotto, Lavigne and Dunyach-Remy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C. elegans L4 host response to S. aureus and H. kunzii infections alone or in association at 12 h post infection. The figure represents the expression of six main genes involved in C. elegans immune response: cyp-37B1 (A), hlh-30 (B), lys-5 (C), sodh-1 (D), clec-7 (E), lgg-1 (F). Data are expressed as the mean ± sd of three biological replicates of qRT-PCR results. All Ct-values are normalized against the housekeeping gene snb-1. Data analysis was performed with the Pfaffl method (Pfaffl, 2001). The P-value represents the comparison between genes expression found in H. kunzii strains alone and the other combination (S. aureus alone, co-infection H. kunzii+S. aureus) ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.