Table 3.

Fifty percentage Lethal Time of Caenorhabditis elegans co-infected with S. aureus strains and two representative H. kunzii strains and evaluation of feeding behavior by measuring the pathogen avoidance.

Strain	LT50 in days (IC95% inf-sup)	Occupancy test after 16h (%)	P OP50 vs. others	P NSA1385 vs. others	P Newman vs. others	P H10 vs. others	PH13 vs. others
NSA1385	4.7 (4.5–4.8)	98 ± 2	<0.001	–	NS	NS	<0.001
Newman	4.3 (4.0–4.6)	95 ± 4	<0.001	NS	–	<0.001	<0.001
H10	5.5 (4.6–6.4)	96 ± 4	<0.001	NS	<0.001	–	NS
H13	6.2 (5.8–6.6)	100 ± 0	NS	<0.001	<0.001	NS	–
H10 + NSA1385	4.0 (3.9–4.2)	100 ± 0	<0.001	NS	–	<0.001	<0.001
H10 + Newman	3.6 (3.5–3.7)	90 ± 5	<0.001	<0.001	NS	<0.001	<0.001
H13 + NSA1385	5.8 (5.7–5.9)	100 ± 0	<0.001	<0.001	–	NS	NS
H13 + Newman	6.3 (6.2–6.4)	90 ± 5	NS	<0.001	<0.001	NS	NS
H10 + OP50	5.6 (5.1–6.0)	100 ± 0	<0.001	NS	<0.001	NS	NS
H13 + OP50	6.4 (6.0–6.6)	100 ± 0	<0.001	<0.001	<0.001	NS	NS
H10> + NSA1385*	4.9 (4.7–5.1)	100 ± 0	<0.001	NS	–	NS	<0.001
H10> + Newman*	4.6 (4.3–4.8)	95 ± 4	<0.001	NS	NS	NS	<0.001
H13> + NSA1385 *	5.2 (5.0–5.3)	100 ± 0	<0.001	NS	–	NS	<0.001
H13> + Newman*	5.5 (5.2–5.7)	92 ± 3	<0.001	NS	<0.001	NS	NS

The results are representative of at least four independent assays for each group of strains. P, Pairwise comparison between LT50s using a log rank test; NS, not significant; LT50, 50% Lethal Time. Infection of nematodes with H10 or H13 followed by transfer on

^*S. aureus 12 h after.