Table 4.

Primers used in the study.

Primer use and target function	Target region	Primer name	Oligonucleotide sequence	Tm (°C)	References
HOST qRT-PCR
Transcriptional factor for host defense	hlh-30	hlh-30 F	5′-CGGGCTGGCTCAGGACACTC-3′	65.5	Visvikis et al., 2014
		hlh-30 R	5′-GGCGCCGAACTTGAGACGAC-3′	63.5
Antimicrobial function	lys-5	lys-5 F	5′-CGGGCTGGCTCAGGACACTC-3′	54.7	Visvikis et al., 2014
		lys-5 R	5′-GGCGCCGAACTTGAGACGAC-3′	53.2
	clec-7	clec-7 F	5′-TTTATGGGACGATTCGACGG-3′	57.3	Visvikis et al., 2014
		clec-7 R	5′-GTCAATGCACCTTGTACGGA-3′	57.3
Cytoprotection	cyp-37B1	cyp-37B1 F	5′-GAATGTATCCGTCAGTGCCA-3′	57.3	Irazoqui et al., 2010
		cyp-37B1 R	5′-TCGGACTCCTTTTGGGAAGA-3′	57.3
Detoxification	sodh-1	sodh-1 F	5′-CTGGATGGCAACTTGGAGACAAAGC-3′	64.6	Irazoqui et al., 2008
		sodh-1 R	5′-GGTGGCAGAGTGGCTCGTGG-3′	65.5
Autophagy	lgg-1	lgg-1 F	5′-ACCATGACCACAATGGGACAACTC-3′	62.7	Visvikis et al., 2014
		lgg-1 R	5′-ACACTTTCGTCACTGTAGGCGATG-3′	62.7
S. aureus qRT-PCR
α hemolysin	hla	Hla-F	5′-TCCAGTGCAATTGGTAGTCA-3′	55.3	Otto et al., 2013
		Hla-R	5′-GGCTCTATGAAAGCAGCAGA-3′	57.3
Protein A	spa	Spa-F	5′-TATGCCTAACTTAAATGCTG-3′	51.1	Otto et al., 2013
		Spa-R	5′-TTGGAGCTTGAGAGTCATTA-3′	53.2
Accessory gene regulator	agrA	F_agrA_34	5′-CAAAGAGAAAACATGGTTACCATTATTAA-3′	58.2	Garzoni et al., 2007
		R_agrA_135	5′- CTCAAGCACCTCATAAGGATTATCAG-3′	61.6