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. 2017 Mar 16;8:448. doi: 10.3389/fmicb.2017.00448

FIGURE 7.

FIGURE 7

Investigation of process stability of immobilized (S)-selective AMDase-CLGIPL on amino C2 acrylate carrier. Reaction conditions: 1 mL reaction volume, 100 g/L amino C2 acrylate carrier with immobilized AMDase mutant, enzyme loading: 23.13 mgenzyme/gcarrier, pH 8, 900 rpm, 30°C. Activity assay: conversion of 25 mM naproxen malonate. The carrier was washed after reaction with distilled water and incubated further in 1 mL water at pH 8.0, 30°C and 900 rpm.