TABLE 1.

Primers

PCR target	Primer	Oligonucleotide sequencea (5′→3′)	Reference	PCR product size (bp)
fliC	FLIC-F	CAAGTCATTAATACAAACAGC	13	1,500
	FLIC-R	TTAACGCAGTAAAGAGAGGAC
fljB	FLJB-F	CAAGTAATCAACACTAACAGT	13	1,500
	FLJB-R	TTAACGTAACAGAGACAGCAC
blaTEM	TEM-F	ATAAAATTCTTGAAGACGAAA	31	1,080
	TEM-R	GACAGTTACCAATGCTTAATC
blaCTX-M consensus	CTX-M-F	CRATGTGCAGYACCAGTAA	This study	540
	CTX-M-R	CGCRATATCRTTGGTGGTG
blaCTX-M-9	CTX-M-9-F	GTGACAAAGAGAGTGCAACGG	27	856
	CTX-M-9-R	ATGATTCTCGCCGCTGAAGCC
gyrA	STGYRA1	TGTCCGAGATGGCCTGAAGC	2	470
	STGYRA12	CGTTGATGACTTCCGTCAG
Class I integron	5′-CS	GGCATCCAAGCAGCAAGC	17	Variable
	3′-CS	AAGCAGACTTGACCTGAT
Region upstream of blaCTX-M-9	ORF513-F	GCAGCACTACCCAGCCTTCA	This study	2,700
	MA-2-R	CCGCRATATGRTTGGTGGTG

^aY, C or T; R, A or G.