Fig. 3.

Melting temperatures of Rh-FGD1 in different buffer (a) and additive (b) conditions measured by the Thermofluor® technique. Buffers were used at a concentration of 100 mm unless otherwise indicated. The error bars represent SD from the three replicates. Buffer A succinic acid/ NaH₂PO₄/ glycine = (2:7:7). Buffer B citric acid/ CHES/ HEPES = (2:4:3). Ac acetate, Am ammonium, DTT dithiothreitol