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. 2016 Dec 13;101(7):2831–2842. doi: 10.1007/s00253-016-8038-y

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Two-substrate kinetic analysis for Rh-FGD1 via double reciprocal plots of reaction rates against a G6P or b F₄₂₀ concentrations. These lines intercept at one point, corresponding to the formation of a ternary complex Rh-FGD1:G6P:F₄₂₀ to generate 6-phosphogluconolactone and F₄₂₀H₂