Table 1.

List of primers used in this study

fgd genes	Forward primers (5′–3′)	Reverse primer (5′–3′)
RHA1_RS43115 (Rh-FGD1)	ATGGTGATCAAGTTCGGGTAC	TCATGCGAGCCCTCGCAG
RHA1_RS10755 (Rh-FGD2)	CTACCCCCGCAGCCG	ATGGCCCACGAACTCAAGC
RHA1_RS43570 (Rh-FGD3)	ATGACACAGCAGTTAAAGCTC	TCAGCCCAGGGCACG
RHA1_RS43115-K197N	TACAACTCCATGCC ATT ACCGGACGTGCAGATG	CATCTGCACGTCCGGT AAT GGCATGGAGTTGTA
RHA1_RS43115-K258N	TGACACCGGAGCAG AAT CATTCGATCGACGATC	GATCGTCGATCGAATG ATT CTGCTCCGGTGTCA
RHA1_RS43115-R282Q	CAGGTGGCGAAG CAG TGGATCGTGGCG	CGCCACGATCCA CTG CTTCGCCACCTG

The mutation sites were indicated as underlined oligonucleotides