Table 3.
Rh-FGD1 | Glucose-6-phosphate | Glucose | ||||
---|---|---|---|---|---|---|
K m [mm] | k cat [s−1] | k cat/K m [m −1 s−1] | K m [mm] | k cat [s−1] | k cat/K m [m −1 s−1] | |
wild type | 0.31 ± 0.016 | 17 ± 0.32 | 57,000 | >300 | >0.015 | 0.056 |
K197N | 95 ± 12 | 3.8 ± 0.29 | 40 | >300 | >0.020 | 0.072 |
K258N | 61 ± 5.1 | 0.57 ± 0.024 | 9.4 | >300 | >0.0009 | 0.0039 |
R282Q | >100 | >0.047 | 0.67 | >300 | >0.0045 | 0.0018 |