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. 2016 Dec 13;101(7):2831–2842. doi: 10.1007/s00253-016-8038-y

Table 3.

Steady-state kinetic parameters for the wild-type Rh-FGD1, K197N Rh-FGD1, K258N Rh-FGD1, and R282Q Rh-FGD1 for G6P and glucose

Rh-FGD1 Glucose-6-phosphate Glucose
K m [mm] k cat [s−1] k cat/K m [m −1 s−1] K m [mm] k cat [s−1] k cat/K m [m −1 s−1]
wild type 0.31 ± 0.016 17 ± 0.32 57,000 >300 >0.015 0.056
K197N 95 ± 12 3.8 ± 0.29 40 >300 >0.020 0.072
K258N 61 ± 5.1 0.57 ± 0.024 9.4 >300 >0.0009 0.0039
R282Q >100 >0.047 0.67 >300 >0.0045 0.0018