Fig. 2.

Effect of concomitant treatment of gastric cancer cells with trastuzumab and chemotherapeutics or cetuximab on cell proliferation. GSU and MKN45 cells were treated for 72 h with 10 µg/ml trastuzumab and/or 10 µg/ml cetuximab (a) or chemotherapeutics (25 ng/ml cisplatin, 2.5 ng/ml 5-FU; b). The metabolic activity of the cells was measured using the WST-1 cell proliferation assay. For concomitant trastuzumab and cetuximab treatment, no increased inhibitory effect in comparison with the monotreatment was observed. However, addition of chemotherapeutics to trastuzumab treatment yielded in GSU cells in an enhanced inhibition of cell proliferation, compared to trastuzumab or chemotherapeutics alone. The mean value of three independent experiments is shown. p values at significance levels of ≤0.050 and ≤0.010 are indicated by (*) and (**), respectively