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. 2017 Mar 15;2(2):e00054-17. doi: 10.1128/mSphere.00054-17

FIG 2 .

FIG 2 

Generation of PruQΔap2IX-4 parasites. (A) Schematic illustrating the generation of the AP2IX-4 knockout by allelic replacement with a DHFR*-TS minigene. (B) Genomic PCRs were performed with the indicated primers (A to G) to verify replacement of the AP2IX-4 genomic locus with the DHFR*-TS minigene. RT-PCR performed with primers B and E confirmed the absence of AP2IX-4 transcripts in PruQΔap2IX-4 parasites. Primers for GAPDH were used as a positive control for the RNA preparation. (C) Representative doubling assay showing the number of parasites/vacuole at 24- and 36-h time points postinfection for parental PruQ versus PruQΔap2IX-4 parasites. Three independent assays were performed with similar results. P > 0.05 (unpaired two-tailed Student’s t test). (D) Plaque assays were performed to compare the in vitro viability of PruQ parasites to that of PruQΔap2IX-4 parasites. Three independent assays were performed with similar results. P = 0.98 (unpaired two-tailed Student’s t test).