FIG 5 .
Loss of AP2IX-4 results in lower frequency of bradyzoite cyst formation in vitro. Parental PruQ, Δap2IX-4, and Δap2IX-4::AP2IX-4HA parasites were cultured in alkaline medium for 4 days and then stained with Dolichos lectin to visualize tissue cyst walls. For each sample, 100 random vacuoles were surveyed for the presence or absence of lectin staining to determine the frequency of cyst formation. n = 4 for parental and Δap2IX-4; n = 3 for Δap2IX-4::AP2IX-4HA. *, P = 0.04; **, P = 0.006 (unpaired two-tailed Student’s t test). Error bars represent standard errors of the means.