TABLE 3.

Dideoxyoligonucleotide primers used for P. aeruginosa MLST

Locus and function	Primer sequence (5′ to 3′)		Amplicon size (bp)
	Forward	Reverse
acsA
Amplification	ACCTGGTGTACGCCTCGCTGAC	GACATAGATGCCCTGCCCCTTGAT	842
Sequencing	GCCACACCTACATCGTCTAT	GTGGACAACCTCGGCAACCT	390
aroE
Amplification	TGGGGCTATGACTGGAAACC	TAACCCGGTTTTGTGATTCCTACA	825
Sequencing	ATGTCACCGTGCCGTTCAAG	TGAAGGCAGTCGGTTCCTTG	495
guaA
Amplification	CGGCCTCGACGTGTGGATGA	GAACGCCTGGCTGGTCTTGTGGTA	940
Sequencing	AGGTCGGTTCCTCCAAGGTC	TCAAGTCGCACCACAACGTC	372
mutL
Amplification	CCAGATCGCCGCCGGTGAGGTG	CAGGGTGCCATAGAGGAAGTC	940
Sequencing	AGAAGACCGAGTTCGACCAT	ATGACTTCCTCTATGGCACC	441
nuoD
Amplification	ACCGCCACCCGTACTG	TCTCGCCCATCTTGACCA	1,042
Sequencing	ACGGCGAGAACGAGGACTAC	TTCACCTTCACCGACCGCCA	366
ppsA
Amplification	GGTCGCTCGGTCAAGGTAGTGG	GGGTTCTCTTCTTCCGGCTCGTAG	989
Sequencing	GGTGACGACGGCAAGCTGTA	TCCTGTGCCGAAGGCGATAC	369
trpE
Amplification	GCGGCCCAGGGTCGTGAG	CCCGGCGCTTGTTGATGGTT	811
Sequencing	TTCAACTTCGGCGACTTCCA	GGTGTCCATGTTGCCGTTCC	441