Table 2.
The whole molecule, heavy chain, and light chain exact masses of originator tocilizumab and HS628 by MS.
| Type | Originator tocilizumab (4 bathes) Mass (Da) |
HS628 (4 bathes) Mass (Da) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| B2014B10 | B2019B26 | B1018B13 | B2027B13 | 20130901 | 20130902 | 20130903 | 20130904 | ||
| Whole molecule |
G0F/G0F | 147885.19 | 147884.44 | 147885.81 | 147885.61 | 147885.78 | 147887.00 | 147882.39 | 147887.77 |
| G0F/G1F | 148046.00 | 148045.88 | 148046.81 | 148046.61 | 148046.27 | 148048.14 | 148044.20 | 148048.41 | |
| G1F/G1F | 148207.06 | 148206.64 | 148207.81 | 148207.63 | 148208.34 | 148208.23 | 148204.53 | 148209.59 | |
| G1F/G2F | 148365.19 | 148364.81 | 148365.66 | 148365.53 | 148366.00 | 148369.11 | 148364.52 | 148371.30 | |
| Deglycosylated molecule | 144998.63 | 144996.91 | 144997.30 | 144997.67 | 144997.02 | 144997.81 | 144994.66 | 144999.08 | |
|
| |||||||||
| Heavy chain |
G0F | 50445.32 | 50445.18 | 50445.46 | 50445.50 | 50443.57 | 50443.88 | 50444.11 | 50443.75 |
| G1F | 50606.94 | 50606.87 | 50606.97 | 50607.01 | 50605.44 | 50605.91 | 50605.71 | 50605.55 | |
| G2F | 50767.95 | 50767.80 | 50767.92 | 50767.96 | 50772.80 | 50772.06 | 50774.06 | 50776.70 | |
| Deglycosylated molecule | 48999.88 | 48999.73 | 48999.92 | 49000.03 | 48997.97 | 48998.05 | 48998.36 | 48998.13 | |
|
| |||||||||
| Light chain | 23500.97 | 23500.96 | 23500.97 | 23500.96 | 23500.75 | 23500.75 | 23500.77 | 23500.76 | |