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. 2017 Mar 14;23(10):1816–1827. doi: 10.3748/wjg.v23.i10.1816

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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miR-34a levels were significantly decreased after oxaliplatin treatment. A: Analysis of miR-34a expression was performed on blood samples from 30 colorectal cancer (CRC) patients after oxaliplatin (OXA)-based combination chemotherapy. Total RNA was extracted and subjected to qRT-PCR to analyze the expression level of miR-34a in each sample. U6 was used as an internal control; B and C: mRNA expression levels of Smad4 and TGF-β in the blood samples from CRC patients were measured by qRT-PCR. β-actin was used as the internal control (^aP < 0.05, ^eP < 0.001); D: The inverse correlation of miR-34a and Smad4 expression levels was examined by Spearman correlation analysis (R = -0.283, P = 0.029).