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. 2017 Mar 14;23(10):1816–1827. doi: 10.3748/wjg.v23.i10.1816

Figure 5.

Figure 5

miR-34a inhibited oxaliplatin-induced autophagy in colorectal cancer cells. A: HT29 and HT29-oxaliplatin (OXA) cells were transfected with miR-34a mimics and treated with or without OXA (10 μmol/L) simultaneously. After 48 h, autophagy was determined using a fluorescent dye (monodansylcadaverine, MDC) and flow cytometry; B: The above cell lysates were examined with indicated antibodies by western blotting. β-actin was used as loading control. (eP < 0.001 indicates a significant difference vs normal control; cP < 0.05 indicates a significant difference vs transfection with miR-34a mimic group).