| Genomic DNA from Step 18 | 50 ng |
| hCas9F primer, 10 μM | 1 μl |
| hCas9R primer, 10 μM | 1 μl |
| ddH20 | to 12.5 μl |
| 2X KAPA HIFI Hot Start ReadyMix | 12.5 μl |
|
| |
| Final Volume | 25 μl |
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