| genomic DNA from Step 36 | 50–100 ng |
| Forward primer, 10 μM | 1 μl |
| Reverse primer, 10 μM | 1 μl |
| ddH20 | to 10 μl |
| 2X Kapa Hifi Hotstart ReadyMix | 10 μl |
|
| |
| Final Volume | 20 μl |
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