ins180 is more frequent among H. pylori isolates from African Americans than from Caucasians. A PCR survey for ins180 in the response regulator jhp0152 promoter region from 36 H. pylori strains from both African Americans (n = 18) and Caucasians (n = 18) was performed. The jhp0153-jhp0152 intergenic region amplicon without ins180 is ∼350 bp, while the amplicons possessing ins180 are 530 bp. Intergenic regions possessing ins480 yield amplicons of ∼830 bp. (A) Lane 1, H. pylori strain Afr7 (containing ins480); lane 2, strain 26695 (no ins180 or ins480); lane 3, strain J99 (containing ins180); lane 4, 100-bp ladder. (B and C) H. pylori strains derived from18 African Americans and 18 U.S. Caucasians, respectively.