TABLE 2.
Virus gene detection of serum samples from confirmed dengue patients by TaqMan RT-PCR and conventional RT-PCR
| Ct range of TaqMan RT-PCR (cycles) | Detection by:
|
||
|---|---|---|---|
| TaqMan RT-PCR | Conventional RT-PCR
|
||
| Type specific | Cross-reactive | ||
| <25 | 10/10 (100)a | 10/10 (100) | 9/9 (100) |
| 25-29.9 | 7/7 (100) | 6/7 (86) | 6/6 (100) |
| 30-34.9 | 7/7 (100) | 6/6 (100) | 7/7 (100) |
| 35-39.9 | 6/6 (100) | 3/4 (75) | 3/6 (50) |
| ≥40 | 5/5 (100) | 2/4 (50) | 1/5 (20) |
| Total | 35/35 (100) | 27/31 (87) | 26/33 (79) |
a
Positive samples/number of tested samples (%).