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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2017 Jan 6;104:20–31. doi: 10.1016/j.freeradbiomed.2017.01.006

Figure 1. LysCN stability under acid hydrolysis and base hydrolysis conditions.

Figure 1

LysCN (1 mM) was incubated with either 6N HCl at 110°C or 4N NaOH at 120°C. At the indicated times, 2-AAA and LysCN content in the reaction were analyzed by LC/MS/MS following solid phase extraction (SPE) as described under Materials and Methods. A, Lysine nitrile (△) is not stable under conventional acid hydrolysis condition and quantitatively decomposes to 2-AAA (○). B, Lysine nitrile (△) is not stable under base hydrolysis conditions and quantitatively decomposes to 2-AAA (○). Data shown represent the mean +/− S.D. of duplicated determinations for a study performed greater than three independent times.