Figure 11. Both 2-aminoadipic acid (2-AAA) and lysine nitrile (LysCN) are enriched in protein recovered from human aortic atherosclerotic tissues compared to normal aorta.
Samples were prepared from normal human aortic tissue (n=5) and atherosclerotic plaque-laden aortic tissue (n=5). Protein bound 2-AAA and LysCN were quantified after protease-mediated hydrolysis by LC/MS/MS mass spectrometric analysis as described under methods. Data points shown represent duplicate analyses of a sample from an individual aorta, reported as μmol oxidation product/mol Lys of either 2-AAA or LysCN, as indicated, and the bar represents mean ± S.D.