Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2017 Jan 6;104:20–31. doi: 10.1016/j.freeradbiomed.2017.01.006

Figure 7. Protein bound 2-AAA and LysCN formation requires protein lysyl residue exposure to the complete MPO/H2O2/Cl− system.

Figure 7

HSA (1 mg/mL, 15 μM) was exposed to the complete MPO/H2O2/Cl− system, or to the indicated components of the MPO system. The production of 2-AAA LysCN and oxidation precursor lysine were quantified by LC/MS/MS as described in Materials and Methods. Only under the complete MPO/H2O2/Cl− system, 2-AAA and LysCN were produced. Data values represent the mean ± S.D. of triplicate samples for an experiment performed at least 3 separate times.