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. 2004 Sep 15;5:20. doi: 10.1186/1471-2172-5-20

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Copyright © 2004 Shen et al; licensee BioMed Central Ltd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FACS-sorting of naïve and memory B-cells from splenocytes. A single B-cell suspension prepared from a fresh spleen was isolated using the Human B-cell Isolation Kit (See methods) and subjected to 3-color cell sorting. Memory B-cells from the splenic B-cells were gated on the IgM^highIgD^lowCD27⁺fraction, while naïve B-cells were gated at IgM^lowIgD^highCD27^-. FACS-sorted populations were over 90 % pure, judging from post sort immunophenotyping.