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. 2017 Mar 16;7:44598. doi: 10.1038/srep44598

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Copyright © 2017, The Author(s)

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The signal peptide and pro-domain are shown in blue and red, respectively. The secondary structure from PtrToxA (PDB entry 1zle) is also shown. Confocal images demonstrating the chloroplast localisation of ToxA_62–132-GFP when transiently expressed in the pavement cells of N. benthamiania leaves. The left panel displays the transmitted light image overlayed with the ToxA_62–132-GFP and RUB1sp-mCherry fluorescence. The middle panel displays the RUB1sp-mCherry fluorescence (chloroplast marker) whilst the right panel displays the ToxA_62–132-GFP fluorescence. Scale bar = 20 μm.