Figure 4. Differential mode of action between α-Syn(A53T) and PTX toward Gi-coupled receptors.

Differentiated HL-60 cells were pretreated with 1 μM α-Syn(A53T) or 100 ng/ml PTX for 18 hr. Upon stimulation by 0.1 μM fMLP, superoxide anion production was measured. Values represent means ± s.e.m. of 3 independent experiments carried out in triplicate. Statistical significance was analysed by Student’s t-test (n = 9; **P < 0.01 versus control cells treated with fMLP).