Table 4. Association (ka₁, ka₂) and dissociation (kd₁, kd₂) rate constants obtained for the HPA and the L1 chip using the two state model.

HPA chip
	ka1	kd1	K1	ka2	kd2	K2	KA
DOPE/DOPG
myxinidin	(3.55 ± 0.12)·101	(3.19 ± 0.14)·10−2	1.1·103	(8.34 ± 0.29)·10−3	(1.63 ± 0.03)·10−3	5.1	5.61·103
WMR	(7.45 ± 0.01)·102	(3.55 ± 0.17)·10−2	2.1·104	(7.94 ± 0.29)·10−3	(5.13 ± 0.12)·10−3	1.5	3.15·104
DOPE/DOPG/CL
myxinidin	(6.64 ± 0.18)·102	(1.33 ± 0.04)·10−1	5.0·103	(5.92 ± 0.08)·10−3	(1.12 ± 0.03)·10−3	5.3	2.65·104
WMR	(5.57 ± 0.02)·102	(1.47 ± 0.03)·10−2	3.8·104	(4.47 ± 0.05)·10−3	(2.33 ± 0.09)·10−3	1.9	7.22·104
L1 chip
DOPE/DOPG/CL
myxinidin	(9.43 ± 0.19)·102	(5.17 ± 0.02)·10−1	1.8·103	(2.13 ± 0.09)·10−3	(1.15 ± 0.03)·10−3	1.8	5.19·103
WMR	(6.98 ± 0.05)·102	(3.53 ± 0.03)·10−1	2.0·103	(2.85 ± 0.04)·10−2	(3.25 ± 0.05)·10−4	88	1.75·105

The affinity constants K₁ and K₂ are for the first (K₁ = k_a1/k_d1) and for the second (K₂ = k_a2/k_d2) steps respectively, and the affinity constant (K_A) determined as (k_a1/k_d1)·(k_a2/k_d2) is for the complete binding process.