Figure 3. Calretinin-expressing interneurons are progressively lost during the symptomatic phase in the SOD1^G93A motor cortex.

(a) CR-expressing neurons were labelled throughout the SOD1^G93A disease course, showing neurons were present at comparable levels in SOD1^G93A and WT mice at 8 weeks (early symptom onset) and 12 weeks in motor (M) and somatosensory cortex (S). (b) Analysis of 16 week symptomatic SOD1^G93A mice showed that CR neurons were significantly decreased in the supragranular lamina of motor cortex (Ms, layers I-IV) compared to WT mice. CR-neurons were progressively reduced in the supragranular lamina of motor cortex (Ms, layers I-IV) in 20 week end-stage SOD1^G93A mice (arrow heads in a). Values in graphs represent means ± SEM. *P < 0.05, two-way ANOVA, Bonferonni’s multiple-comparison test with n = 6 mice per group. Scale bar in (a) 50 μm.