Figure 1. Skeletal muscle-enriched lnc-mg is induced during myogenesis.

(a) Microarray heat map of annotated lncRNAs in undifferentiated MuSCs (growth media, GM) and 5-day differentiated MuSCs (differentiation media, DM). (b) Real-time PCR analysis of lnc-mg expression in mouse tissues. Mean values±s.e.m., n=6, *P<0.05. Mice were 8 weeks old, three for male, three for female. (c) Real-time PCR analysis of lnc-mg expression in mouse skeletal muscles and other major organs. Mean values±s.e.m., n=6. Mice were 8 weeks old, three for male, three for female. (d) Real-time PCR analysis of lnc-mg expression in MuSCs during 5 days of differentiation. Mean values±s.e.m., n=3. (e) lnc-mg associated gene subsets were determined by Gene Ontology analysis. The data statistical significance is assessed by Student's t-test.