Fig. 1.

Growth-phase dependency of PPK and PPX in B. cereus. With a 1% inoculum of an overnight culture, B. cereus was grown in LB media at 37°C with vigorous shaking. At the indicated time points (corresponding to middle-log, late-log, early-stationary, and late-stationary phases), cells were harvested, suspended in TED buffer (50 mM Tris·HCl, pH 7.4/1 mM EDTA/1 mM DTT), and treated with lysozyme and sonication to give crude lysates for enzyme assays (see Materials and Methods).