Fig. 5.

Abiotic surface attachment of WT and mutants. An overnight LB culture (2 μl) was inoculated into 200 μl of LB in 96-well polystyrene plates and incubated at 30°C without shaking. After a 12-h incubation, the media were removed, each well was rinsed with sterile water, and the surface attachments were stained with crystal violet, extracted, and measured as described in Materials and Methods.