Fig. 6.

Sporulation of WT and mutants. (A) Sporulation efficiency. Cultures were grown in CCY sporulation medium. Samples at the indicated times were diluted, heated at 70°C for 15 min, and plated on LB agar. Spore frequency was determined by the population that survived the heat treatment and was counted as colonies the next day. (B) PPK, PPX enzymatic activities, and the poly-P level in the sporulating WT cells. Enzyme assays and poly-P determination were performed as described in Materials and Methods.