Table 2. Normalized Kd values for R2 mutants binding to GH/R1 complexes.
| Mutation | hGHv | POH4 | POH1 | WT hGH* |
|---|---|---|---|---|
| R43A-R2 | 1.0 | 0.5 | 2 | |
| E44A-R2 | 0.3 | 0.2 | 1 | |
| S102A-R2 | 15.0 | 15.0 | 13 | |
| I103A-R2 | 8.0 | 4.0 | 5 | |
| W104A-R2 | NB | NB | NB | NB |
| D126A-R2 | 33.0 | 30.0 | 37 | |
| I165A-R2 | 1.0 | 1.0 | 2 | |
| Q166A-R2 | 1.0 | 1.0 | 1 | |
| W169A-R2 | NB | 61.0 | 64 | 11 |
| W104F-R2 | 125.0 | 37.0 | 56 | 81 |
| W169F-R2 | 3.0 | 6.0 | 3 | 6 |
Values are normalized to Kd values for hGHv, POH4, POH1, and WT hGH of 4.4, 2.1, 4.1, and 3.8 nM, respectively. POH1 contains the following mutations: F10A, M14W, H18D, and H21N. NB, no binding (Kd > 1,000 nM).
*
Values taken from Walsh et al. (23).