Table 3. Normalized pairwise Kd values for GH 116 mutants and R2 Trp mutants.
| Variants | WT R2 | W104F-R2 | W169F-R2 |
|---|---|---|---|
| D116A-HGHv | 61 | NB (7,600) | NB (183) |
| D116A-POH4 | 12 | NB (444) | 68 (72) |
| D116A-hGH* | 1 | NB (66) | 3 (4) |
| D116N-hGHv | 6 | 28 (750) | 17 (18) |
| D116N-POH4 | 3 | 68 (11) | 21 (18) |
| D116N-hGH* | 3 | NB (243) | 15 (14) |
| D116V-hGHV | 9 | 76 (1,100) | 28 (27) |
| D116V-POH4 | 7 | NB (259) | 39 (42) |
| D116V-hGH* | 2 | NB (162) | 24 (9) |
Values are normalized to the Kd values for hGHv, POH4, and WT hGH of 4.4, 2.1, and 3.8 nM, respectively. NB, no binding (Kd > 1,000 nM). Values in parentheses are calculated binding energy additivities based on the single-point mutations: Kd(Mutant1)/Kd(Ref)·Kd(Mutant2)/Kd.
*
Values taken from Walsh et al. (23).