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. 2017 Mar 15;7:44452. doi: 10.1038/srep44452

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Copyright © 2017, The Author(s)

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Decline in the survival of B. subtilis culture after incubation with various concentrations of CSA-13 (panels A–C) evaluated using a killing assay method. Level of metabolic activity observed in ceragenin-treated spore samples exposed to appropriate environmental conditions when compared to untreated control (panels D–F) assessed using the MTT assay. Samples were incubated at RT (panels A, D for vegetative form and panels B, E for spore form, respectively) and at 70 °C (panels C, F) for 15 (white squares), 30 (grey circles), 60 (black circles), 120 (white triangles) and 240 (grey diamonds) minutes. Data from experiments performed in triplicate are shown.