Figure 2. Measurement of threshold cycle (Ct) values of Imjin virus (MJNV) RNA genome in MJNV- infected Crocidura lasiura tissues.

RT-qPCR was performed to amplify the MJNV M segment in C. lasiura tissues, including lungs, livers, kidneys, and spleens. IFA⁺ PCR⁺ indicates anti-MJNV IgG seropositive and MJNV RNA positive shrews (n = 3); IFA⁻ PCR⁺ indicates anti-MJNV IgG seronegative and MJNV RNA positive shrews (n = 6). The vertical axis shows the Ct value for amplification of the MJNV M segment.