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. 2017 Mar 10;8:14666. doi: 10.1038/ncomms14666

Figure 5. Sema7A is required for the functional integration of adult-born DG granule cells.

Figure 5

(a) Representative images of an MMLV CAG-GFP infected adult-born granule cell (nGC) used for recording of electrophysiological properties and miniature excitatory post-synaptic currents (mEPSCs). Healthy cells were identified using differential interference contrast (DIC). Recording of nGCs (GFP-positive cells) was confirmed by the presence of recording pipet-dye Alexa Fluor 594 in the same cell. (b) Representative traces of membrane potential (upper traces) in response to current injections (lower traces) in Sema7A+/+ (WT, black) or Sema7A−/− (KO, blue) nGCs. Note the reduced hyperpolarization of the membrane potential during hyperpolarizing current injections in KO nGCs. (c) Representative traces of action potentials in WT and KO nGCs. (d) Representative traces for action potential firing rates (upper trace) in response to a 200 pA current injection (lower trace) in WT and KO nGCs. (e) Representative traces of mEPSC recordings in WT and KO nGCs. Asterisks indicate identified mEPSC events. (f–i) Quantification of mEPSC instantaneous frequency (f), amplitude (g), rise- (h) and decay- (i) time (n(WT)=9 cells/3 mice, n(KO)=8 cells/3 mice, Student's t-test). Data are presented as means±s.e.m. **P<0.01. Scale bars: (a) 10 μm, (b,d) horizontal: 220 ms, vertical: 50 mV (upper traces) or 200 pA (bottom traces), (c) horizontal: 2 ms, vertical: 20 mV, (e) horizontal: 200 ms, vertical: 10 pA.