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. 2017 Mar 10;8:14666. doi: 10.1038/ncomms14666

Figure 7. Sema7A promotes neurite growth from differentiated adult SGZ neuronal progenitors in vitro.

Figure 7

(a) Immunocytochemistry for Tuj1 and glial fibrillary acidic protein (GFAP) on cultures of differentiated SGZ neuronal progenitors at 3 days post-differentiation in the presence of 2 nM Fc control or Sema7A-Fc protein. (b) Quantification of the number of GFAP-negative/Tuj1-negative, GFAP-positive (astrocytes) or Tuj1-positive (neurons) cells in cultures of differentiated SGZ neuronal progenitors. Addition of Sema7A-Fc does not alter the relative number of neurons and astrocytes in the cultures. (c) Immunocytochemistry for microtubule-associated protein 2 (Map2) to label dendrites on cultures of differentiated SGZ neuronal progenitors at 10 days post-differentiation in the presence of 2 nM exogenous Fc control or Sema7A-Fc protein in the presence of a β1-integrin blocking antibody or control IgG1 antibody. (d–f) Quantification of total Map2+ neurite length (d), longest Map2+ neurite length (e) or the number of Map2+ branch points (f) in cultures as in c (two-way ANOVA, post-hoc Replicate-paired t-test). Data represent means±s.e.m. from at least thre independent experiments. *P<0.05 and **P<0.01. Scale bars: 50 μm.