Figure 1. Generation of Foxo1/3/4 liver-specific knockout mice (LTKO).

(A–C) Real-time PCR analysis of Foxo1, Foxo3, and Foxo4 mRNAs in the WT and LTKO mouse livers (n = 3), respectively *p < 0.05. (D) Western blot analysis of Foxo1 and Foxo3 proteins in WT and LTKO livers. The blots for the proteins shown are in the cropped format, and the full-length blots are presented in the Supplementary Figure 1. As Foxo4 protein is barely detectable in the mouse liver, Foxo4 Western blot data were not presented.