. 2017 Mar 16;7:44644. doi: 10.1038/srep44644

Table 1. Primer sets used for RT-PCR characterization of CHEST cells.

Gene	Definition	Forward primer (5′-3′)	Reverse primer (5′-3′)	Annealing temperature (°C)	Product size (bp)
tie2 (tek)	TEK tyrosine kinase, endothelial	GAAGTGTGTGTGTGCAAAAG	CAGTGTTGATCTCCACATCC	55	134
kdr	kinase insert domain receptor	TCAGACTCAGAGCTCTTGAG	GACTATAGTAGGGGTCGGTG	55	122
tpo (thpo)	thrombopoietin	ATGGAAAGCAACGTGACTGG	CCACTGCAGATTACCTTTTC	55	178
tnfa	tumor necrosis factor a	GAGAGATCGCATTTCACAAG	TTCCTCAGTCAGTTCAGACG	55	230
kdrl³¹	kinase insert domain receptor like	GCCTCGGGTCAATGCTGTTCC	GTCCGGTTGCCAAGTTCATTCC	60	561
flt1³¹	fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)	ATCCGCAGTGTTATATTCCTCCAT	TTGCGCTCCTTTTGCCGATACCT	60	410
angpt1³¹	angiopoietin 1	CTACAACAGAGCGCCGTCCAC	AGGTCCTGCTGTCTCTGAAG	60	419
fli1a	fli-1 proto-oncogene, ETS transcription factor a	ATGGACGGAACTATTAAGGAGGC	TTAGTAGTAACTACCAAGGTGTG	60	1355
pecam1³¹	platelet/endothelial cell adhesion molecule 1	GACGGGCACGCTGATGTTTCTCTTC	CTGCACGCTCCCTTTCTCGGTTTTG	60	427
cdh5³¹	cadherin 5	CCCCGTTTTCGATTCTGACC	CTTTGAGGCTTAGCATTCCATCTT	60	515
acta1a¹⁶	actin, alpha 1a, skeletal muscle	GAAAAGAGCTACGAGCTTCC	GTAAGTGGTCTCGTGAATGC	50	129
myod1¹⁶	myogenic differentiation 1	ATGGCATGATGGATTTTATG	TTTATTATTCCGTGCGTCAG	50	107
actc1a¹⁶	actin, alpha, cardiac muscle 1a	TGCTGTCTTTCCCTCTATTG	GAGTGAGGATACCCCTCTTG	50	116
acta2¹⁶	actin, alpha 2, smooth muscle, aorta	TGGATCTGGACTGTGTAAGG	ACTATCTTTCTGCCCCATTC	50	121
gata1a⁴⁹	GATA binding protein 1a	TGAATGTGTGAATTGTGGTG	ATTGCGTCTCCATAGTGTTG	55	650
cd45 (ptprc)⁴⁹	protein tyrosine phosphatase, receptor type, C	AGTTCCTGAAATGGAAAAGC	GCACAGAAAAGTCCAGTACG	55	140
ef1a (eef1a1l1)⁴⁹	eukaryotic translation elongation factor 1 alpha 1, like 1	GAGAAGTTCGAGAAGGAAGC	CGTAGTATTTGCTGGTCTCG	55	123
epo¹⁸	erythropoietin	ACTTGTAAGGACGATTGCAG	TATCTGTAATGAGCCGATGG	55	156
gcsfa (csf3)¹⁰	colony stimulating factor 3a, granulocyte colony stimulating factor	AACTACATCTGAACCTCCTG	GACTGCTCTTCTGATGTCTG	55	165
gcsfb (csf3b)¹⁰	colony stimulating factor 3b, granulocyte colony stimulating factor b	GGAGCTCTGCGCACCCAACA	GGCAGGGCTCCAGCAGCTTC	55	184
il-11a	interleukin 11a	GACAAGCTGAGCAATCAGAC	GGAGCTGAGAAAGAGTAGGC	50	172
il-11b	interleukin 11b	TTGAACATTCGCTATCATCC	GAGTAATCGTTCCCCAATTC	50	166
m17¹⁶	il-6 subfamily cytokine M17	CTTGATTGCCGTTCAGTTAG	TGACCGGAGATTGTAGACAC	50	210
fgf1¹⁸	fibroblast growth factor 1	ATACTGCGCATAAAAGCAAC	AGTGGTTTTCCTCCATCTTC	50	154
fgf21¹⁸	fibroblast growth factor 21	CGGTGGTGTATGTATGTTCC	GTAGCTGCACTCTGGATGAC	50	203
kitlga¹⁸	kit ligand a	GGATTCAATGCTTGACTTTG	TGTACTATGTTGCGCTGATG	50	205
kitlgb¹⁶	kit ligand b	GGCAACCAGTCCACCAATAAG	CACTTTTCCCTTCTGTAGTGGC	50	135
vegfaa¹⁶	vascular endothelial growth factor Aa	GAAACGTCACTATGGAGGTG	TTCTTTGCTTTGACTTCTGC	50	121
bmp1l¹⁸	bone morphogenic protein 1, like	GGATGGATATTGGAGGAAAG	CTTTGTTCGGTCTGTAATCG	50	230
cxcl12a¹⁶	chemokine (C-X-C motif) 12a, stromal cell-derived factor 1a	CGCCATTCATGCACCGATTTC	GGTGGGCTGTCAGATTTCCTTGTC	50	297
cxcl12b¹⁶	chemokine (C-X-C motif) 12b, stromal cell-derived factor 1b	CGCCTTCTGGAGCCCAGAGA	AGAGATTCTCCGCTGTCCTCC	50	291
il-1b¹⁶	interleukin 1, beta	TCCACATCTCGTACTCAAGG	CAGCTCGAAGTTAATGATGC	50	227
ifnɣ¹⁶	interferon, gamma 1-2	TACATAATGCACACCCCATC	TCCTTTGTAGCTTCATCCAC	55	158
il-26¹⁸	interleukin 26	TGAAAAGATGTGGGATGAAC	ACTGATCCACAGCAAAACAC	55	214
il-15¹⁸	interleukin 15, like	CCAAGTCCACAATTACATGC	TCTTTGTAGAGCTCGCAGAC	55	166
il-12a¹⁸	interleukin 12a	GTGAGTCTGCTGAAGGAGTG	AGTGACATCATTTCCTGTGC	50	167
il-1018	interleukin 10	ATGAATCCAACGATGACTTG	TCTTGCATTTCACCATATCC	50	222
il-22	interleukin 22	CTACCTGCGATATGAAGTGC	GAAATATGGAAGCAGTCGTG	50	171
dla¹⁸	δA	ACGACGATTTGAGTATGACG	GGGATTGGCACTTTATATCC	50	186
dlb¹⁸	δB	TTCCGTGTTTAATGATTTGG	CACTCCACAGAAACTCTTGC	50	158
dlc¹⁸	δC	TGGTGGACTACAATCTGAGC	ACCTCAGTAGCAAACACACG	50	169
dld¹⁶	δD	AACCCAGACCGTCTGATCAGT	CCGGGTTTGTCGCAAAAGCCA	50	308
dll4¹⁸	δ-like 4	CTCTTTCAGCACACCAATTC	TGAACATCCTGAGACCATTC	50	189
jag1a¹⁸	jagged 1a	TGATTGGTGGATACTTCTGC	AATCCATTGAGTGTGTCCTG	55	238
jag1b¹⁸	jagged 1b	CTGTGAGCCATCTTCTTCAG	AGCAAAGGAACCAGGTAGTC	55	213
jag2¹⁸	jagged 2	AATGACTGTGTGAGCAATCC	GTCATTGACCAGATCCACAC	50	174
mpx⁵⁰	myeloid-specific peroxidase	TGATGTTTGGTTAGGAGGTG	GAGCTGTTTTCTGTTTGGTG	55	158
pax5	paired box 5	GACCCTCTCCAGGATTCTCC	TGGGGTTTGACCAGGAAATA	60	501
lck	LCK proto-oncogene, Src family tyrosine kinase	GCCAGAGGGGTTACATACCA	TGATAGCCACTTGACGGTTG	60	501
cmyb (myb)	v-myb avian myeloblastosis viral oncogene homolog	AAGACCAACGGGTGATTGAG	CAGTGCCATGTTGTGGAAAG	60	507
cd41 (itga2b)⁴⁹	Integrin, alpha 2b	CTGAAGGCAGTAACGTCAAC	TCCTTCTTCTGACCACACAC	55	197
band3 (slc4a1a)	solute carrier family 4 (anion exchanger), member 1a (Diego blood group)	CAACTTCCAGAAGAAGATG	TTGACCATACCACCAAATG	55	144
igm (ighm)⁵⁰	immunoglobulin heavy constant mu	AGCTTCTCTAGCTCCACCAG	ATTTTGGTGAAATGGAATTG	55	477