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. 2017 Mar 10;8:14729. doi: 10.1038/ncomms14729

Figure 3. Chlamydia-induced cell death is not apoptotic or necroptotic.

Figure 3

(a) Time-lapse videomicroscopy of mCherry-Rab25 stable HeLa cells ablated 24 h p.i. with CTL2 (MOI∼0.5) in the presence of CellEvents Caspase-3/7 Green Detection Reagent. (b) Quantification of inclusion rupture induced cell death under the indicated conditions. N=10 biological replicates for each condition. (c) Quantification of native egress-induced cell death under the indicated conditions. Presented in pie-charts are the proportions of surviving primary infected, secondary infected and uninfected cells at the indicated time-point as monitored by GFP-CTL2 fluorescence. N=3 biological replicates with >200 cells counted per replicate. Error bars present the s.d. from the mean. For clarity, only error bars and P values for DMSO and chloramphenicol are presented. **P≤0.01; (Unpaired Student's t-test). (d) Western blotting of HeLa cells that had been genome-edited for the indicated targets full blots are shown in Supplementary Fig. 5.