Fig. 3.
Hepatocarcinogenesis from Apc–/– hepatocytes. (A and B) HCC1 and HCC2 developed 8 and 9 months, respectively, after AdCre infection. (C) PCR analysis from tumoral DNA shows the loss of the Apclox allele and the gain of the ApcΔex14 allele. (D–F) MD HCCs. (G–I) PD HCCs. Hematoxylin/eosin staining shows atypical hepatocytes organized in a trabecular (D) or pseudoglandular (G) pattern; β-catenin mislocalizes in the nucleus (E and H), and GS is overexpressed (F and I). Nontumoral (NT) and tumoral (T) tissues are delineated by a dashed line. (J and K) GS immunostaining in macronodular HCCs (J) and in a micronodular preneoplastic lesion (K), indicated by arrows. (Scale bars: A and B, 1 cm; D–I, 50 μm; J and K, 1 mm.)