Table 1. Reconciled macroscopic growth parameters of Rol-producing and reference strain in glycerol:methanol chemostat cultures.
qs, glycerol (mmol g−1 DCW h−1) | qs, methanol (mmol g−1 DCW h−1) | qCO2 (mmol g−1 DCW h−1) | qO2 (mmol g−1 DCW h−1) | DCW (g−1 L−1) | Yx/s (g−1 DCW C-mol−1) | Residual methanol (g/L) | Residual glycerol (g/L) | |
---|---|---|---|---|---|---|---|---|
0C | −1.12 ± 0.01 | −2.01 ± 0.11 | 2.03 ± 0.10 | 3.52 ± 0.14 | 12.82 ± 0.16 | 16.84 ± 0.53 | 0.11 ± 0.10 | 0.04 ± 0.02 |
1C | −1.20 ± 0.03 | −2.22 ± 0.10 | 2.35 ± 0.13 | 3.98 ± 0.06 | 12.03 ± 0.11 | 15.97 ± 0.18 | 0.10 ± 0.09 | 0.03 ± 0.01 |
2C | −1.51 ± 0.03 | −1.59 ± 0.14 | 2.28 ± 0.09 | 4.16 ± 0.24 | 8.99 ± 0.15 | 14.35 ± 0.08 | 4.39 ± 0.28 | 0.04 ± 0.01 |
4C | −1.71 ± 0.09 | −0.62 ± 0.21 | 2.08 ± 0.28 | 3.82 ± 0.31 | 8.66 ± 0.41 | 16.18 ± 1.04 | 7.07 ± 0.19 | 0.03 ± 0.03 |
8C | −1.75 ± 0.05 | −0.67 ± 0.12 | 1.97 ± 0.08 | 3.75 ± 0.06 | 8.26 ± 0.10 | 15.37 ± 0.14 | 7.52 ± 0.23 | 0.03 ± 0.1 |
15C | −1.73 ± 0.12 | −1.08 ± 0.24 | 2.18 ± 0.16 | 4.05 ± 0.23 | 8.55 ± 0.34 | 14.92 ± 0.13 | 5.90 ± 0.92 | 0.02 ± 0.02 |
Substrate consumption and biomass production rates for each strain. Consistency index h was below 5 in all cases (95% confidence level). Data are shown as means ± standard deviation based on three independent experiments for each strain. qs: reconciled substrate consumption specific rate; qCO2: CO2 exchange rate; qO2: O2 oxygen uptake rate; YX/S: reconciled biomass/substrate yield.